Cell Cycle · 2004 · 37 citations · 0 references
Life SciencesBiochemistryMolecular BiologyCell SignalingMolecular Cell BiologyProtein-protein InteractionsCell Signaling NetworksCyclin-dependent KinasesMacromolecular AssemblyCyclin RegulationCyclin-dependent Kinase ComplexesCyclin-dependent Kinase Signaling PathwaysCyclinsEvolutionary Conservation Of DomainsHydrophobic InteractionsMitotic CyclinsProtein Binding Motif
We recently reported that the 'hydrophobic patch' (HP) of the Saccharomyces cerevisiae S-phase cyclin Clb5 facilitates its interaction with Orc6 (via its Cy or RXL motif), providing a mechanism that helps prevent rereplication from individual origins. This is the first finding of a biological function for an interaction between a cyclin and a cyclin-binding motif (Cy or RXL motif) in a target protein in Saccharomyces cerevisiae. It is also the first such example involving a B-type cyclin in any organism. Yet, some of our observations as well as work from other groups suggest that HP-RXL interactions are functionally important for cyclin-Cdk signaling to other targets. The evolutionary conservation of the HP motif suggests that it allows cyclins to carry out important and specialized functions.