Gene cloning of an NADPH-dependent menadione reductase from Candida macedoniensis, and its application to chiral alcohol production

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Life Sciences, Molecular Biology, Molecular Genetics +12

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

Annie Chang, Stanley N. Cohen · Journal of Bacteriology · 1978 · 4.3K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Genetic applications of an inverse polymerase chain reaction.

Howard Ochman, Anne S. Gerber, Daniel L. Hartl · Genetics · 1988 · 1.9K citations · Full text

Life Sciences, Genetics, Molecular Genetics +11

Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by Escherichia coli transformant cells coexpressing the aldehyde reductase and glucose dehydrogenase genes

Michihiko Kataoka, K. Yamamoto, Hiroshi Kawabata et al. · Applied Microbiology and Biotechnology · 1999 · 143 citations

Life Sciences, Biochemistry, Microbiology +14