Microbiology and Immunology · 1996 · 24 citations · 10 references
MedicineInfectious DiseasesMicrobiologyDiagnostic MicrobiologyInfectious Disease EpidemiologyMedical MicrobiologyBacterial EpidemiologyFoodborne PathogensGastrointestinal InfectionsInfectious DiarrheaMicrobial ContaminationPathogen DetectionAntiserum PreparationClostridial Toxin DetectionClostridium PerfringensClostridium Perfringens InfectionElderly PopulationGel ElectrophoresisLatex Agglutination AssaysLatex Agglutination Tests
To diagnose sporadic diarrhoea due to Clostridium perfringens infection, faecal specimens from elderly patients were examined directly for C.perfringens enterotoxin using reverse passive latex agglutination assay, and then cultured for this organism. C.perfringens isolates from those samples were grouped by slide agglutination and by pulsed-field gel electrophoresis (PFGE). Fifty of the 60 isolates agglutinated with newly raised antiserum WX2 and 38 shared the same genomic PFGE pattern. Characteristics of the epidemics and experimental data suggest that the diarrhoea was caused by a nosocomial spread of C.perfringens, and not by a food-borne outbreak.
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Manual of Clinical Microbiology
Jacob Blair, E H Lennette, J P Truant · Postgraduate Medical Journal · 1970 · 2.7K citations · Full text