Biphasic Radiosensitization of Human Lymphocytes by Diethyldithiocarbamate: Possible Involvement of Superoxide Dismutase

Abstract

A biphasic radiosensitization of human lymphocytes by diethyldithiocarbamate (DDC), a metal chelator, was observed. The first phase occurred at 10(-5) M and the second at 10(-3) MDDC. The biphasic radiosensitization coincided with the previously reported biphasic toxicity of DDC. Inhibition of superoxide dismutase (SOD) occurred only in the second phase, suggesting that it may be a contributing cause of this phase. The mechanism of the first radiosensitization phase is not known. The radiation survival curves indicated the presence of at least two lymphocyte populations differing in their radiosensitivity and representing 40 per cent and 60 per cent of the cells. Both cell populations were biphasically radiosensitized by DDC.