Cytochemical Identification of Monocytes and Granulocytes

TLDR

These cytochemical methods are simple, sensitive, and reproducible. The study discusses the specific aims and potential clinical applications of these cytochemical methods. Cytochemical assays employing chloroacetate esterase, nonspecific esterase, peroxidase, and metachromasia detect monocytes and neutrophilic, basophilic, or eosinophilic granulocytes, producing highly chromogenic, solvent‑insoluble products that can be used alone or in combination to identify specific cell types.

Abstract

Cytochemical methods for chloroacetate esterase, nonspecific esterase, peroxidase, and metachromasia were used to identify monocytes and neutrophilic, basophilic, or eosinophilic granulocytes. These methods are very simple, sensitive, and easily reproducible. Their reaction products are highly chromogenic and insoluble in most organic solvents. Any of these methods may be used independently or in combination with another cytochemical method to demonstrate a particular type of cell as desired. The specific aims and possible areas for clinical application of these cytochemical methods are discussed.