Science · 1998 · 319 citations · 11 references
Life SciencesCell BiologyMolecular BiologyCell CycleCell DivisionDna ReplicationCell Cycle ControlCell-cycle CheckpointsCell-cycle RegulationDna Replication ControlMitosisReplication Checkpoint ControlCell Division ControlCheckpoint Kinase SignalingReplication Checkpoint ActivationReplication Checkpoint SignalingWee1 InhibitionWee1 Kinase InhibitionWee1 Kinase RegulationWee1 Kinase Signaling
Cdc2, the kinase that induces mitosis, is regulated by checkpoints that couple mitosis to the completion of DNA replication and repair. The repair checkpoint kinase Chk1 regulates Cdc25, a phosphatase that activates Cdc2. Effectors of the replication checkpoint evoked by hydroxyurea (HU) are unknown. Treatment of fission yeast with HU stimulated the kinase Cds1, which appears to phosphorylate the kinase Wee1, an inhibitor of Cdc2. The protein kinase Cds1 was also required for a large HU-induced increase in the amount of Mik1, a second inhibitor of Cdc2. HU-induced arrest of cell division was abolished in cds1 chk1 cells. Thus, Cds1 and Chk1 appear to jointly enforce the replication checkpoint.
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Cdc25 Mitotic Inducer Targeted by Chk1 DNA Damage Checkpoint Kinase
Beth Furnari, Paul Russell · Science · 1997 · 541 citations