Structure determination of the O‐specific polysaccharides from <i>Citrobacter</i> O4‐ and O27‐lipopolysaccharides by methylation analysis and one‐ and two‐dimensional ¹ H‐NMR spectroscopy

Abstract

Using sugar and methylation analyses, and one- and two-dimensional 1H-NMR spectroscopy at 500 MHz it was established that poly-beta-1,2-4-deoxy-D-arabinohexopyranose occurs as O-specific chains of lipopolysaccharides in Citrobacter serotypes O4, O27 and O36. Strong serological cross-reactivity between these serotypes is in full agreement with the chemical identity of their O-specific polysaccharides.