Correlation of Cellular Aggregation of Transformed Cells with Their Growth in Soft Agar and Tumorigenic Potential

Abstract

Formation of cellular aggregates by rat, mouse, hamster, and human transformed cells was compared to aggregate formation by untransformed control cells. Viability and proliferation of cells in the aggregate form were found to correlate well with tumorigenic potential regardless of the method of transformation (spontaneous, chemical, or virus-induced). This assay has the potential for a fast and accurate means of evaluating in vitro transformation.