Overcoming antibody expression and screening limitations by smart design: Applications to PSA immunoassay development

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

George P. Smith · Science · 1985 · 4.1K citations

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Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

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A Decade of Development in Immunoassay Methodology

J. P. Gosling · Clinical Chemistry · 1990 · 459 citations · Full text

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Production of recombinant proteins by high cell density culture of Escherichia coli

Jong Hyun Choi, Ki Chang Keum, Sang Yup Lee · Chemical Engineering Science · 2005 · 342 citations

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Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent—application to a human single-chain Fv fragment

Kouhei Tsumoto, Katsutoshi Shinoki, Hidemasa Kondo et al. · Journal of Immunological Methods · 1998 · 196 citations

Life Sciences, Biochemistry, Molecular Biology +7