Syntheses of Nucleosides Designed for Combinatorial DNA Sequencing

Abstract

Fluorescent and photolabile nucleotide analogues 1 and 2 were prepared to test an unconventional approach to DNA sequencing that, ideally, would not involve gel electrophoresis. These particular compounds were not incorporated, but modeling studies indicate how further refinements to their structures could facilitate the proposed sequencing scheme. B = adenine, thymine; TP = triphosphate,