Hematological and physiological changes induced by short-term exposure to copper in the freshwater fish, Prochilodus scrofa

Abstract

Hematological and physiological changes in the blood of juveniles of the freshwater fish, Prochilodus scrofa were determined after acute exposure to 20, 25, and 29 microgramsCu L-1 in water (pH 7.5; hardness 24.5 mg L-1 as CaCO3) for 96 h. Copper exposure to 25 and 29 microgramsCu L-1 caused significant increase in the hematocrit and red blood cell values. The increase in red blood cells was associated with increase in whole blood hemoglobin only in fish exposed to 29 microgramsCu L-1. Leukocytes increased following copper exposure and were significantly higher in fish exposed to 29 microgramsCu L-1. Differential leukocyte percentage displayed significant reduction in lymphocytes and an increase in neutrophils in fish exposed to 25 and 29 microgramsCu L-1. The percentage of monocytes remained unchanged after copper exposure. The thrombocytes did not change. There was a significant decrease in plasma [Na+] and [Cl-] and a significant drop in blood pH in fish exposed to 25 and 29 microgramsCu L-1 while [K+] showed significant increase in fish exposed to 29 microgramsCu L-1. Copper exposure led to ionoregulatory impairment, although chloride cell hypertrophy was induced. The changes in red blood cells suggest a compensatory response to respiratory surface reduction of gills (tissue damage and cell proliferation) in order to maintain oxygen transference from water to the tissues, allowing the fish to survive during the so-called shock phase of LC50 exposure, at least while at rest.