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A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS
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Tissue EngineeringEngineeringCell CultureBiomedical EngineeringCell GrowthCellular PhysiologySkin RegenerationRegenerative MedicineSpherical FormMatrix BiologyLiving CellsTissue BiologyXenotransplantationThe Fixed TissuesCell ManipulationCell BiologyFresh PlasmaTissue CultureMedicineTissue CellsHuman TissueExtracellular Matrix
Individual, living tissue cells can be obtained in suspension by digesting growing tissue cultures with trypsin, producing spherical cells. The study aims to address the problems opened up by this method. After washing and plating in fresh plasma, cells extend processes and proliferate, and the digestion and plating can then be repeated after growth. The method’s limits have not yet been reached.
Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new plates has occurred the digestion and plating can be repeated. The limits of the method have not yet been reached. We are at work on a number of the problems which it has opened up.