Plant Signaling & Behavior · 2012 · 40 citations · 28 references
Life SciencesMolecular BiologyPlant BiologyHost-pathogen InteractionsInnate ImmunityPlant Molecular BiologyPlant Disease ResistancePlant Immune ResponsesPlant Virus ResistancePlant VirusesPlant-pathogen InteractionsVirus-host InteractionsCysteine ProteasesEffector Recognition By Host PlantsPlant Defense MechanismsPlant Defense SuppressionPlant Pathogen EffectorsRna Silencing SuppressionSilencing Suppressor ProteinsViral Rna Silencing Suppressors
The V2 protein of Tomato yellow leaf curl geminivirus (TYLCV) is an RNA-silencing suppressor that counteracts the innate immune response of the host plant. However, this anti-host defense function of V2 may include targeting of other defensive mechanisms of the plant. Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. This binding occurred both in vitro and in vivo, within living plant cells. The V2 binding site within mCYP1 was identified in the direct proximity to the papain-like cysteine protease active site.
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