Magnetic Resonance in Chemistry · 1998 · 76 citations · 0 references
Natural SciencesChemistryAnalytical ChemistryChromatographic AnalysisSpectroscopyChromatographyLiquid ChromatographyMagnetic Resonance SpectroscopySolvent ExtractionConcentrationSignal-to-noise RatioSignal-to-noise Ratio AnalysisSignal-to-noise Ratio AssessmentSignal-to-noise Ratio EnhancementSignal-to-noise Ratio ImprovementSolvent OptimizationSpectral Overlap Correction
Significant gains in LC–NMR signal-to-noise ratio are obtained by selective concentration of chromatographic peaks on a chromatographic column using D2O to fix the sample on the solid phase. A deuterated organic solvent is used to remove the sample from the column and into the NMR flow cell, which removes the need for presaturation of solvent resonances. Deuterated solvents unrelated to the original eluent are used in order to minimize spectral overlap between solvent and solute. © 1998 John Wiley & Sons, Ltd.