Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection

Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

F W Alt, Rodney E. Kellems, Joseph R. Bertino et al. · Journal of Biological Chemistry · 1978 · 980 citations · Full text

Life Sciences, Genetics, Molecular Genetics +4

Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.

G Urlaub, Lawrence A. Chasin · Proceedings of the National Academy of Sciences · 1980 · 910 citations

Life Sciences, Genetics, Molecular Genetics +9

Activation of the Complement System by Synthetic DNA Complexes: A Potential Barrier for Intravenous Gene Delivery

Christian Plank, Karl Mechtler, Francis C. Szoka et al. · Human Gene Therapy · 1996 · 583 citations

Pharmacy, Immunology, Molecular Pharmaceutics +17

Fusion of cationic liposomes with mammalian cells occurs after endocytosis

Iwona Wrobel, David S. Collins · Biochimica et Biophysica Acta (BBA) - Biomembranes · 1995 · 339 citations

Life Sciences, Biophysics, Cell Biology +14

Nonbilayer phase of lipoplex–membrane mixture determines endosomal escape of genetic cargo and transfection efficiency

Inge S. Zuhorn, Udo Bakowsky, Evgeny Polushkin et al. · Molecular Therapy · 2005 · 264 citations · Full text

Pharmacy, Molecular Pharmaceutics, Pharmaceutics +10