Spleen-Colony Formation in Anemic Mice of Genotype <i> WW ^V </i>

Abstract

The hemopoietic cells from anemic mice of genotype WW are less able by 200-fold to take part in colony formationin the spleen than cells from the normal littermates of genotype ww. The genetic defect shows itself in the colony-forming cells, since cells from normal littermate mice formcolonies in the spleens of unirradiated mice of genotype WW. Use of animals of genotype WVVWT as recipients improves the spleen-colony method by removing bias resulting fromthe death of irradiated recipients.