Molecular Self‐Assembly, Chemical Lithography, and Biochemical Tweezers: A Path for the Fabrication of Functional Nanometer‐Scale Protein Arrays

Abstract

Electron-induced chemical lithography combined with self-assembled monolayers and multivalent chelators for high-affinity capturing of His-tagged proteins are used to obtain specific, stable, highly parallel, and functional protein micro- and nanoarrays on solid substrates. The functionality of the generated large-area protein arrays is shown in situ via specific, homogeneous, oriented and reversible immobilization of His6-tagged 20S proteasome and fluorescence labelled His10-tagged maltose binding proteins. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2008/c2189_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.