Configuration‐dependent Raman bands of phospholipid surfaces: 2—Head group and acyl stretching modes in the 800–900 cm⁻¹ region

Abstract

Abstract Deuteration studies, hydration studies, melting studies and model compound studies are utilized to assign and interpret the 800–900 cm −1 region of the Raman spectra of the phospholipids dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolamine. Head group Raman bands in this region are shown to be sensitive to hydration and melting. The acyl carbon‐carbon stretches, assigned to Raman bands appearing at 860–890 cm −1 , are shown to be sensitive probes of molecular packing order, specifically in the acyl interface region.