Molecular Endocrinology · 1987 · 42 citations · 0 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionProtein TranslationSignal TransductionProtein SynthesisTranslation RegulationCdna CloningCdna Library ConstructionCdna SynthesisHeat Shock FactorsHeat Shock Protein RegulationHeat Shock ResponseHeat Shock Response GenesRegulation By Heat Shock Proteins
A cDNA clone, pNb29, was isolated from a library made from mRNA of Nb2 rat lymphoma cells stimulated by PRL. The nucleotide sequence of pNb29 was found to be identical to the 70-kilodalton heat-shock-like (hsp-70-like) mRNA. The levels of this mRNA increased 8 +/- 3-fold after PRL stimulation of arrested Nb2 cells, and is expressed in different amounts in the normal rat tissues analyzed. To determine whether hsp-70-like mRNA was PRL responsive in vivo, ovine PRL was administered to hypophysectomized rats. A 5 +/- 2-fold increase in hepatic hsp-70-like mRNA was observed 9 h after injection. Thus PRL appears to regulate a heat-shock-like mRNA both in vitro and in vivo. This novel finding extends the already wide range of biological effects ascribed to PRL.