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Studies of metalloprotein species by directly coupled high-performance liquid chromatography inductively coupled plasma mass spectrometry
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1987
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EngineeringBiological Mass SpectrometryChemistrySpectrochemical AnalysisChemical EngineeringEnvironmental ChemistryGas ChromatographyEnvironmental Analytical ChemistryBioanalysisPlasma Mass SpectrometryAnalytical ChemistryLiquid ChromatographyProteomicsElemental CharacterizationChromatographyBiochemistryHplc-icp-ms CouplingsMetalloprotein SpeciesChromatographic AnalysisBiomolecular EngineeringHigh-performance Liquid ChromatographyNatural SciencesSpectroscopyMetalloproteinMass SpectrometryProtein Mass SpectrometryNative Mass SpectrometryTheoretical Plates
High-performance liquid chromatography (HPLC) has been directly coupled to inductively coupled plasma mass spectrometry (ICP-MS) for trace metal speciation studies. The characteristics of HPLC-ICP-MS couplings have been investigated using the number of theoretical plates, peak tailing, rise time and wash-out time as criteria of merit. A short aerosol connection line was found to be optimal. In comparison with an in-line UV monitor, ICP-MS was found to give a comparable number of theoretical plates.