Native mass spectrometry is an analytical mass spectrometry technique employed for the characterization of biomolecules, especially proteins and their non-covalent complexes, under conditions designed to maintain their native-like structure and interactions in the gas phase. This approach involves electrospraying samples from non-denaturing solutions, enabling the intact transfer of macromolecular assemblies into the vacuum for mass analysis, thereby providing critical insights into their stoichiometry, higher-order structure, and interactions vital for structural biology and biophysics.