N-glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

Life Sciences, Biotechnology, Genetics +17

Functional profiling of the Saccharomyces cerevisiae genome

Guri Giaever, Angela Chu, Li Ni et al. · Nature · 2002 · 4.5K citations

Life Sciences, Genetics, Molecular Biology +13

A general method of<i>in vitro</i>preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

Russell Higuchi, Barbara Krummel, Randall K. Saiki · Nucleic Acids Research · 1988 · 2.4K citations · Full text

Life Sciences, Genetics, Molecular Biology +12

The Molecular Chaperone Calnexin Binds Glc1Man9GlcNAc2 Oligosaccharide as an Initial Step in Recognizing Unfolded Glycoproteins

Felecia E. Ware, Aikaterini Vassilakos, Per A. Peterson et al. · Journal of Biological Chemistry · 1995 · 419 citations · Full text

Life Sciences, Biochemistry, Cell Biology +15

Human Insulin from Recombinant DNA Technology

Irving S. Johnson · Science · 1983 · 401 citations

Life Sciences, Bioengineering, Biotechnology +17