Emerging infectious diseases · 2006 · 53 citations · 24 references
AgriculturePoultry ScienceVeterinary MicrobiologyPoultry MedicinePoultry MicrobiologyPoultry PathologyBacterial Diseases Of PoultryBroiler BreedingPoultry BacteriologyPoultry DiagnosticsPoultry EpidemiologyAmplified Fragment Length Polymorphism AnalysisAmplified Fragment Length Polymorphism MarkersBroiler ChickensBroiler Health Risk AssessmentHelicobacter ZoonosesMicrobiological DiagnosticsMolecular Typing Of Pathogens
A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single strain that disseminates throughout the flock. Strains isolated from different hosts or geographic sources displayed a distinctive pattern. H. pullorum is present in approximately one third of live chickens in Belgium and may represent a risk to human health.
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AFLP: a new technique for DNA fingerprinting
Pieter Vos, René C. J. Hogers, Marjo Bleeker et al. · Nucleic Acids Research · 1995 · 12.4K citations · Full text
Kurt Houf, Ann Tutenel, Lieven De Zutter et al. · FEMS Microbiology Letters · 2000 · 275 citations