Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.

TLDR

The study used high‑speed printed glass microarrays of 1,046 human cDNAs and a two‑color hybridization assay to quantitatively monitor differential gene expression, with differentially expressed spots subsequently sequenced. The assay identified both known and novel heat‑shock and phorbol‑ester‑regulated genes in human T cells, demonstrating its sensitivity and enabling rapid, large‑scale human gene discovery.

Abstract

Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.