Journal of Basic Microbiology · 1994 · 11 citations · 12 references
Life SciencesBiotechnologyMicrobiologyEnzymologyFermentation ScienceMicrobial MetabolismBacterial FermentationEnzyme ProductionFermentation KineticsFood FermentationMicrobial Enzyme ProductionMicrobial GrowthAerationAlkaline FermentationAmylase ProductionEnzyme Production OptimizationEnzyme Yield OptimizationTemperature Effects On Fermentation KineticsTemperature OptimumThermostable Amylases
Abstract Bacillus stearothermophilus AN 002 secreted thermostable alkaline amylase into a liquid growth medium containing 2 g/l starch. Amylase activity was highest at early stationary phase of growth. Among the various carbohydrates tested maximum amylase production was obtained with starch. When starch was replaced by other carbon sources, amylase production was reduced. Peptone and corn steep liquor (CSL) were the ideal nitrogen sources for amylase production by this strain. Of the phosphate sources tested, (NH 4 ) 2 HPO 4 showed best restults. Amylase production was highest in a laboratory fermenter at a initial pH of 6.5 and at a constant pH of 7.0. The optimum aeration and agitation for amylase production were 0.66 v/v/m and 400 rev/min, respectively. Maximum growth was obtained at 55 °C and maximum amylase was produced at 50 °C. The amylase was found to be stable between pH 6.0 to 11.0.
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