Porcine <i>PPARGC1A</i> (peroxisome proliferative activated receptor gamma coactivator 1A): coding sequence, genomic organization, polymorphisms and mapping

Abstract

We report here the characterisation of porcine <i>PPARGC1A</i>. Primers based on human <i>PPARGC1A</i> were used to isolate two porcine BAC clones. Porcine coding sequences of <i>PPARGC1A </i>were sequenced together with the splice site regions and the 5′ and 3′ regions. Using direct sequencing nine SNPs were found. Allele frequencies were determined in unrelated animals of five different pig breeds. In the MARC Meishan-White Composite resource population, the polymorphism in exon 9 was significantly associated with leaf fat weight. <i>PPARGC1A</i> has been mapped by FISH to SSC8p21. A (CA)<sub>n</sub> microsatellite <i>(SGU0001)</i> has been localised near marker <i>SWR1101 </i>on chromosome 8 by RH mapping and at the same position as marker <i>KS195</i> (32.5 cM) by linkage mapping. The <i>Ase</i>I (nt<sub>857</sub>, Asn/Asn<sub>489</sub>) polymorphism in exon 8 was used to perform linkage analysis in the Hohenheim pedigrees and located the gene in the same genomic region. Transcription of the gene was detected in adipose, muscle, kidney, liver, brain, heart and adrenal gland tissues, which is in agreement with the function of PPARGC1A in adaptive thermogenesis.