Determination of polar lipids: Quantitative column and thin‐layer chromatography

Abstract

Abstract The structures of the polar lipid classes of plants and animals are presented, their nomenclature discussed, and suggestions are presented for clarification of nomenclature. The three general types of quantitative chromatographic procedures (column chromatography, thin‐layer chromatography, and combinations of column and thin‐layer chromatography) available for polar lipids are reviewed and a new quantitative two‐dimensional thin‐layer chromatographic procedure is presented. Useful quantitative procedures employing columns of cellulose, silicic acid, silicic acid mixed with silicate, magnesium silicate, and ion exchange celluloses are presented. New findings with diethylaminoethyl cellulose columns are described. New quantitative procedures employing silicic acid, magnesium silicate, or diethylaminoethyl cellulose column chromatography with quantitative thin‐layer chromatography are described.