Publication | Open Access
A general method for the purification of restriction enzymes
314
Citations
2
References
1978
Year
Abbreviated ProcedureGeneticsDna AnalysisMolecular BiologyMolecular GeneticsProtein PurificationRestriction EndonucleasesBiosynthesisDna SequencingBiochemistryMolecular Biological MethodOligonucleotideDna ReplicationRestriction EnzymesNatural SciencesBiotechnologyGenetic EngineeringEnzyme SpecificityProtein EngineeringSufficient PurityMedicineGenome Editing
An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.
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