Nano Letters · 2006 · 83 citations · 42 references
EngineeringBioengineeringBiomedical EngineeringElectrophysiologyMicroelectromechanical SystemsMicrofabricationBiomedical InstrumentationMicroelectrode ArraysMicrofluidic DevicesMicrofluidic SystemsMicrofluidicsPatch-clamp ElectrophysiologyChannelsMicrofabrication Of Electrode ArraysMicrofluidic PlatformsPatch Clamp TechniquesPatch-clamp InstrumentationPatch-clamp RecordingRecordingsValves
We present a microfluidic system integrated with disposable cell interface partitions for simultaneous patch clamp recordings. Glass-supported poly(dimethylsiloxane) (PDMS) partitions, having a 2 microm air-blown aperture, were reversibly sealed to a microfluidic system including PDMS channels with isolation valves and microfabricated Ag/AgCl electrodes. Gigaseal recordings from RBL-1 cells were obtained with a 24% success rate. Simultaneous whole cell recordings from valve-isolated electrodes were obtained.
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Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography
Marc Unger, Hou-Pu Chou, Todd Thorsen et al. · Science · 2000 · 4K citations
Single-channel currents recorded from membrane of denervated frog muscle fibres
Erwin Neher, Bert Sakmann · Nature · 1976 · 2.5K citations