Nuclear magnetic resonance spectroscopy and reversed‐phase high‐performance liquid chromatography of peracetylated digalactosyldiacylglycerols

Abstract

Abstract A simple method for the peracetylation of digalactosyldiacylglycerols is described. The peracetylated compounds were isolated by thin‐layer chromatography and subjected to nuclear magnetic resonance spectroscopy for structure elucidation. The proton signals at acetylated sites are shifted downfield compared to the nonderivatized compounds, resulting in improved resolution. The peracetylated compounds were also subjected to molecular species analysis by reversed‐phase high‐performance liquid chromatography. The chromatography behavior of the peracetylated compounds was similar to that of triacylglycerols, displaying narrow and symmetrical peaks yielding a highly resolved molecular species profile.