Degradation of HSA-AX15(R13K) when expressed in Pichia pastoris can be reduced via the disruption of YPS1 gene in this yeast

High efficiency transformation by electroporation of <i>Pichia pastoris</i> pretreated with lithium acetate and dithiothreitol

Shixuan Wu, Geoffrey J. Letchworth · BioTechniques · 2004 · 375 citations · Full text

Life Sciences, Biochemistry, Biotechnology +13

Ciliary neurotrophic factor activates leptin-like pathways and reduces body fat, without cachexia or rebound weight gain, even in leptin-resistant obesity

Philip Lambert, Karen E. Anderson, Mark W. Sleeman et al. · Proceedings of the National Academy of Sciences · 2001 · 305 citations · Full text

Life Sciences, Neuroscience, Physiology +16

High-level expression of recombinant human serum albumin from the methylotrophic yeast Pichia pastoris with minimal protease production and activation

Kaoru Kobayashi, Shinobu Kuwae, Tomoshi Ohya et al. · Journal of Bioscience and Bioengineering · 2000 · 196 citations

Life Sciences, Biotechnology, Microbiology +15

Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae

Hyun Ah Kang, Eui‐Sung Choi, W.-K. Hong et al. · Applied Microbiology and Biotechnology · 2000 · 88 citations

Life Sciences, Biochemistry, Molecular Biology +12

Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. Similarity to the mammalian pro-opiomelanocortin-converting enzyme

Anahit V. Azaryan, M Wong, Theodore C. Friedman et al. · Journal of Biological Chemistry · 1993 · 76 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16