Applied and Environmental Microbiology · 2007 · 28 citations · 35 references
Life SciencesGeneticsImmunologyHost-pathogen InteractionsImmunogeneticsMicrobial GeneticsFungal PathogenesisResistance Gene DiscoveryYeast GeneticsHeterologous Expression SystemsHeterologous Gene ExpressionPathogen Resistance GenesResistance Mechanism ProfilingToxin FunctionToxin GenesToxin Identification
The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells.
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