Growth characteristics of reovirus type 2: actinomycin D and the synthesis of viral RNA.

Abstract

type 2, and subsequent conjugation with fluorescein isothiocyanate (FITC) of the antibody- containing globulin fraction were described elsewhere.7Enzymes: The RNase (5X crystallized), DNase I, and chymotrypsin were obtained from Calbiochem, Los Angeles, California.The nucleases were used at the following concentrations for cytological studies: RNase-0.02% in phosphate buffered saline, pH 7.2, for 60 min at 370C DNase-0.01% in phosphate-buffered saline containing 0.003 M Mg++ for 60 min at 370C.