Oligodeoxyribonuclsotide-3′-phosphate Synthesis by Selective Cleavage of 3′-Terminal Uridine

Abstract

Abstract A universal method is evolved for synthesizing oligodeoxyribonucleotides containg a 3′-terminal phosphate group. This method consists of obtaining oligodeoxyribonucleotides containing a 3′-terminal uridine (incorporated by enzyme TdT-catalyzed reaction or by routine triester technique), in selective oxidation of the 3′-terminal ribose of uridine and subsequent β-elimination of the phosphate residue.