Engineering of Bacillus lipase by directed evolution for enhanced thermal stability: effect of isoleucine to threonine mutation at protein surface

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

Life Sciences, Molecular Biology, Molecular Genetics +11

Factors enhancing protein thermostability

Sandeep Kumar, C J Tsai, Ruth Nussinov · Protein Engineering Design and Selection · 2000 · 985 citations · Full text

Life Sciences, Biochemistry, Biophysics +16

ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling

Manuel C. Peitsch · Biochemical Society Transactions · 1996 · 941 citations

Life Sciences, Biochemistry, Molecular Biology +7

Protein thermal stability, hydrogen bonds, and ion pairs

Gerhard Vogt, Stefanie Woell, Patrick Argos · Journal of Molecular Biology · 1997 · 741 citations

Natural Sciences, Biochemistry, Biophysics +16

A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction

DW Leung, E Chen, Goeddel Dv · Medical Entomology and Zoology · 1989 · 612 citations

Life Sciences, Genetics, Molecular Biology +13