Quantitative Determination of Some Constituent Lipids in Human Dentin

Abstract

A method is described for the extraction of dentin lipids and the separation of these lipids into six components (cholesterol esters, free cholesterol, triglycerides, diglycerides, monoglycerides, and phospholipids). Essentially, the method requires the decalcification of dentin using EDTA and subsequent extraction with diethyl ether, ethanol, and chloroform. The lipid extract is separated into six components by a small silicic acid column and the effluents are quantitated colorimetrically.