A SIMPLE METHOD FOR RAPID SCREENING OF BACTERIA WITH GLUTAMATE DECARBOXYLASE ACTIVITIES

Abstract

ABSTRACT A pH indicator method (PIM) for rapid screening of bacteria with glutamate decarboxylase (GAD) activities was established. In this article, 48 different bacterial strains were used for the detection of GAD activities by the PIM, as well as by paper chromatography and liquid chromatography/mass spectrometry. Similar results were obtained by the three methods; however, the PIM is a more simple, rapid and repeatable one.