CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression

Abstract

BackroundCAP (CEVEC's Amniocyte Production) cells are an immortalized cell line based on primary human amniocytes.They were generated by transfection of these primary cells with a vector containing the functions E1 and pIX of adenovirus 5. CAP cells allow for competitive stable production of recombinant proteins with excellent biologic activity and therapeutic efficacy as a result of authentic human posttranslational modification.In order to gain access to the benefits of the CAP technology also for early research, target evaluation or assay development, the transient expression system CAP-T was developed.CAP-T cells are based on the original CAP cells and additionally express the large T antigen of simian virus 40 (SV40).