Enantiomeric separation of dansyl amino acids using MECC with a ligand exchange mechanism

Abstract

Enantiomeric separation of 11 dansyl amino acids is reported using micellar electrokinetic capillary chromatography. The chiral phase consisted of a copper complex of N, N-di-decyl-D-alanine dissolved in the running buffer. Several factors affecting the separation were studied. The partitioning of the copper complex between the micelles and the buffer interface was investigated, and an attempt to describe the mechanism of separation is presented. © 1996 John Wiley & Sons, Inc.