Photoacoustic spectroscopy applied to the study of the influence of laser irradiation on corn seeds

Abstract

In the present study we were interested in the effects of low intensity laser irradiation on hybrid corn seeds CL 1 x CL 4 when these seeds were exposed to different laser intensities and irradiation times. In order to observe qualitative differences in chlorophyll a and b optical absorption spectra of seedling's leaves, whose seeds were irradiated and non irradiated, were obtained by using photoacoustic spectroscopy (PAS). A randomized complete blocks experimental design with three replications was used. The experimental unit included 10 seeds, from which we randomly choose three seedlings. The variance analysis (ANOVA) for both chlorophylls revealed significant (P< 0.05) differences among treatments.