A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

[6] Use of T7 RNA polymerase to direct expression of cloned genes

F. William Studier, Alan H. Rosenberg, John J. Dunn et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 1990 · 6.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +10

Identification of a protein required for disulfide bond formation in vivo

James C.A. Bardwell, Karen McGovern, J Beckwith · Cell · 1991 · 987 citations

Life Sciences, Biochemistry, Molecular Biology +12

Use of Peptide Libraries to Map the Substrate Specificity of a Peptide-Modifying Enzyme: A 13 Residue Consensus Peptide Specifies Biotinylation in Escherichia coli

Peter J. Schatz · Nature Biotechnology · 1993 · 589 citations

Life Sciences, Biochemistry, Molecular Biology +17

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

Claude V. Maina, Paul Riggs, Barton E. Slatko et al. · Gene · 1988 · 540 citations

Life Sciences, Biochemistry, Microbiology +15