Phospholipids. 2. Estimation of amino nitrogen in intact phospholipids

Abstract

may well be some additional steric and ionization effects to be considered which would account for these differences in equivalence of acid groups and albumin molecules. SUMMARY 1. Polyacids interact with albumin in salt-free solutions to prevent coagulation of the protein by heat. Polyphosphates (nucleic acids), polysulphates (heparin and dextran sulphate) and polyearboxylates (alginic acid and bacterial polyglutamic acid) were all effective. Effects on optical rotation of the albumin were also demonstrated.