Science · 1988 · 321 citations · 41 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationGene Regulatory ElementsImmune Cell SignalingOncogenic VirusesPromoter RegulationTranscription FactorsTranscriptional Regulation In EukaryotesImmunoglobulin Gene ExpressionNf-kappa B-dependent TranscriptionNuclear Factor Kappa B ActivationNuclear Factor Kappa B ModulationNuclear Factor Kappa B SignalingProtein-dna InteractionsRegulatory Protein-dna InteractionsTranscriptional Activator Recruitment
Expression of the interleukin-2 receptor (IL-2R alpha) gene is activated by the transcriptional activator protein, Tax (previously referred to as the tat gene product), encoded by the human T-cell leukemia virus (HTLV-I). Multiple protein binding sites for specific DNA-protein interactions were identified over the upstream IL-2R alpha transcriptional regulatory sequences. However, only one region, which includes the sequence motif GGGGAATCTCCC, was required for activation by both the tax gene product and mitogenic stimulation. Remarkably, this sequence also bound the nuclear factor NF kappa B, which is important for induction of kappa-immunoglobulin gene expression. A model is presented whereby regulation of cellular gene expression by the HTLV-I tax gene product occurs via an indirect mechanism that may involve a post-translational modification of preexistent cellular transcription factors.
41
Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences
Julian Downward, Yosef Yarden, E. L. V. Mayes et al. · Nature · 1984 · 2.5K citations · Full text