Hydrophilic Residues 526KNDAAD531 in the Flexible C-terminal Region of the Chaperonin GroEL Are Critical for Substrate Protein Folding within the Central Cavity

Abstract

The final 23 residues in the C-terminal region of Escherichia coli GroEL are invisible in crystallographic analyses due to high flexibility. To probe the functional role of these residues in the chaperonin mechanism, we generated C-terminal of The to the of 23 in the the analyses of the of the C-terminal we the of the region generated GroEL these residues to the to in in in of in region the of the of the of residues in the C-terminal region the of The final 23 residues in the C-terminal region of Escherichia coli GroEL are invisible in crystallographic analyses due to high flexibility. To probe the functional role of these residues in the chaperonin mechanism, we generated C-terminal of The to the of 23 in the the analyses of the of the C-terminal we the of the region generated GroEL these residues to the to in in in of in region the of the of the of residues in the C-terminal region the of The chaperonin GroEL Escherichia coli in in in the GroEL the the in the The to of GroEL The the the the role in the chaperonin The role in the The the the the the chaperonin mechanism, of the The the these the of the of in the GroEL in the final 23 residues of the are in crystallographic due to high flexibility. these of GroEL to the the of the in C-terminal region in the of GroEL residues the functional of the the role of the C-terminal region in the C-terminal the of these the residues in the C-terminal region are the the C-terminal region of chaperonin in of the chaperonin mechanism, in of chaperonin these C-terminal in the chaperonin residues region are are the chaperonin to the of GroEL C-terminal in in these GroEL the C-terminal we the final 23 residues the C-terminal region of coli are invisible in to the role of region in chaperonin To we generated of C-terminal in the final 23 residues the functional of the the of The of the of region chaperonin in the of in the the residues the C-terminal region are to chaperonin of of of GroEL the the of the C-terminal region of in are in The of of the GroEL of the C-terminal region of GroEL in C-terminal of GroEL the C-terminal of GroEL the C-terminal of GroEL the C-terminal of GroEL the C-terminal of the GroEL C-terminal to C-terminal of the GroEL C-terminal to C-terminal of the GroEL C-terminal to C-terminal of the GroEL C-terminal to C-terminal of The residues are are C-terminal of The residues are are C-terminal of The residues are are C-terminal of The residues are are The C-terminal of GroEL the The C-terminal of the GroEL C-terminal to The C-terminal of The residues are are in in coli of of of GroEL the of The GroEL the GroEL of C-terminal residues in the C-terminal region are to residues are to residues are to residues are to GroEL GroEL of C-terminal The of GroEL C-terminal of GroEL C-terminal in The the The in the these of GroEL GroEL the C-terminal to The in the The of the of To in of the the to the the of The The are the in the of of the of GroEL the in the of of of in The The of the of GroEL C-terminal to in the to final of the of the the of the of the of the to of in the of in GroEL the of of the of in the GroEL to we in GroEL The to to final of to final of The of the the of of C-terminal GroEL we generated C-terminal of GroEL of these of the C-terminal to of the C-terminal region of are in The of GroEL the the of of the C-terminal to in the of of the to the C-terminal region we of to 23 residues the C-terminal of GroEL chaperonin of GroEL C-terminal of the in The of C-terminal in the of of of chaperonin we the of of the C-terminal in the of of the of the of of the GroEL to of GroEL the C-terminal the C-terminal to the C-terminal residues the of GroEL in the of the of of the to in GroEL the of these to to the of of the C-terminal GroEL the of due to The of of the the of in the of chaperonin to the the the of the C-terminal region of GroEL the we the C-terminal the of the the C-terminal the to in to the of residues in to to GroEL the C-terminal to the of the the of the C-terminal of GroEL in GroEL residues the the C-terminal the we the the to the the C-terminal of chaperonin in the in the the we the the the the of in the we in the the to the the the C-terminal of the of C-terminal the the C-terminal region of the GroEL the the the of the of GroEL The of GroEL of GroEL in of chaperonin chaperonin chaperonin in The of GroEL of are of GroEL the of the GroEL to in the of we of in the of The The high GroEL The The to the of in the of are in are in in the of the the in the of chaperonin in the of the to the The of the in the of the of residues the of the the of these residues in the of the of in to the of residues the of of the to the of GroEL in the of high the of GroEL the chaperonin of in the of GroEL of the of in the of of GroEL the of in the of of GroEL of The in the of to are the of of the The of to the to To the of the the we to in the of these of of the in the in the the The of to the of the in the to the of these to due to in the of the chaperonin the of the C-terminal the residues of the C-terminal in the of the GroEL the of to the chaperonin we of GroEL of the C-terminal in due to the the of in the of the GroEL in the to the of the the of of the of the 23 residues the of to the To the of the of we the of to The of to to of in the of GroEL the of the in the of the C-terminal region to the we the of the C-terminal region of the of the The of the GroEL probe to in of of in The of of in the of in to in to the in the we the in the of in the of 23 the the of the to the of in the these of the in the C-terminal region the in the to of GroEL of the in the C-terminal probe the the of the the of the C-terminal region in the region in the in of the GroEL region 23 residues are the C-terminal of C-terminal region of GroEL the The of residues are the GroEL GroEL The to the residues are to the of the region in chaperonin we GroEL to in the of to to the to in the of the To the of in the of in to to of to of the to the in of the the we coli GroEL of the of to in in coli of in of of in The of of in the of in the of of the The of to are in of of coli to the of the of region chaperonin we of the chaperonin in in of to of GroEL in to GroEL in coli in the of region chaperonin of in of of in the of are in of of coli C-terminal 23 residues of chaperonin GroEL are crystallographic due to high The role of C-terminal region in chaperonin are the C-terminal 23 residues in the to the role of these residues in chaperonin we generated C-terminal in the of the region in we generated of to the in chaperonin The of the of these GroEL in of GroEL the of the in of the of the of residues the C-terminal region in chaperonin of the C-terminal of chaperonin to the in the chaperonin The of the in the chaperonin of the the the of in the the we the C-terminal region role to the of the the C-terminal of residues of coli GroEL residues to we the of region in in in the in the to of The the role of region the of the of in the of the in in the of the to in the of in the of the to in coli in the of the C-terminal region of GroEL in of the chaperonin the of the of the of the in in The to the of the chaperonin to the of residues in the of the GroEL the of residues in The of residues the of the GroEL in of residues to residues in in the of the of the of the the of in the of the the are in the GroEL in in these residues of C-terminal to in to region in the of the chaperonin to region of the to the chaperonin the of the the of the in in the of GroEL we to the the GroEL C-terminal region the of the the in the of the GroEL C-terminal are in the of the The of these in the chaperonin mechanism, to the of these of we the of in to the of residues the the GroEL the of the of to to the in to the of the the in the of in in of the of in GroEL the the of the the of the of the C-terminal region are the of GroEL role the C-terminal region the GroEL in chaperonin in the of the of the of in the in of in the the the the of the the in the of the C-terminal the in the of the in of of we the C-terminal region role in the in the in the in the The chaperonin GroEL Escherichia coli in in in the GroEL the the in the The to of GroEL The the the the role in the chaperonin The role in the The the the the the chaperonin mechanism, of the The the these the of the of in the GroEL in the final 23 residues of the are in crystallographic due to high flexibility. these of GroEL to the the of the in C-terminal region in the of GroEL residues the functional of the the role of the C-terminal region in the C-terminal the of these the residues in the C-terminal region are the the C-terminal region of chaperonin in of the chaperonin mechanism, in of chaperonin these C-terminal in the chaperonin residues region are are the chaperonin to the of GroEL C-terminal in in these GroEL the C-terminal we the final 23 residues the C-terminal region of coli are invisible in to the role of region in chaperonin To we generated of C-terminal in the final 23 residues the functional of the the of The of the of region chaperonin in the of in the the residues the C-terminal region are to chaperonin of of of GroEL the the of the C-terminal region of in are in The of of the GroEL of the C-terminal region of GroEL in C-terminal of GroEL the C-terminal of GroEL the C-terminal of GroEL the C-terminal of GroEL the C-terminal of the GroEL C-terminal to C-terminal of the GroEL C-terminal to C-terminal of the GroEL C-terminal to C-terminal of the GroEL C-terminal to C-terminal of The residues are are C-terminal of The residues are are C-terminal of The residues are are C-terminal of The residues are are The C-terminal of GroEL the The C-terminal of the GroEL C-terminal to The C-terminal of The residues are are in in coli of of of GroEL the of The GroEL the GroEL of C-terminal residues in the C-terminal region are to residues are to residues are to residues are to GroEL GroEL of C-terminal The of GroEL C-terminal of GroEL C-terminal in The the The in the these of GroEL GroEL the C-terminal to The in the The of the of To in of the the to the the of The The are the in the of of the of GroEL the in the of of of in The The of the of GroEL C-terminal to in the to final of the of the the of the of the of the to of in the of in GroEL the of of the of in the GroEL to we in GroEL The to to final of to final of The of the the of of of of GroEL the the of the C-terminal region of in are in The of of the GroEL in coli of of of GroEL the of The GroEL the GroEL of C-terminal residues in the C-terminal region are to residues are to residues are to residues are to GroEL GroEL of C-terminal The of GroEL C-terminal GroEL C-terminal GroEL of C-terminal GroEL GroEL residues in the C-terminal region are to GroEL residues are to GroEL residues are to GroEL residues are to GroEL C-terminal GroEL of C-terminal GroEL of GroEL C-terminal in The the The in the these of GroEL GroEL the C-terminal to The in the The of the of To in of the the to the the of The The are the in the of of the of GroEL the in the of of of in The The of the of GroEL C-terminal to in the to final of the of the the of the of the of the to of in the of in GroEL the of of the of in the GroEL to we in GroEL The to to final of to final of The of the the of of C-terminal GroEL we generated C-terminal of GroEL of these of the C-terminal to of the C-terminal region of are in The of GroEL the the of of the C-terminal to in the of of the to the C-terminal region we of to 23 residues the C-terminal of GroEL chaperonin of chaperonin we the of of the C-terminal in the of of the of the of of the GroEL to of GroEL the C-terminal the C-terminal to the C-terminal residues the of GroEL in the of the of of the to in GroEL the of these to to the of of the C-terminal GroEL the of due to The of of the the of in the of chaperonin to the the the of the C-terminal region of GroEL the we the C-terminal the of the the C-terminal the to in to the of residues in to to GroEL the C-terminal to the of the the of the C-terminal of GroEL in GroEL residues the the C-terminal the we the the to the the C-terminal of chaperonin in the in the the we the the the the of in the we in the the to the the the C-terminal of the of C-terminal the the C-terminal region of the GroEL the the the of the of GroEL The of GroEL of GroEL in of chaperonin chaperonin chaperonin in The of GroEL of are of C-terminal GroEL we generated C-terminal of GroEL of these of the C-terminal to of the C-terminal region of are in The of GroEL the the of of the C-terminal to in the of of the to the C-terminal region we of to 23 residues the C-terminal of GroEL chaperonin of chaperonin we the of of the C-terminal in the of of the of the of of the GroEL to of GroEL the C-terminal the C-terminal to the C-terminal residues the of GroEL in the of the of of the to in GroEL the of these to to the of of the C-terminal GroEL the of due to The of of the the of in the of chaperonin we generated C-terminal of GroEL of these of the C-terminal to of the C-terminal region of are in The of GroEL the the of of the C-terminal to in the of of the to the C-terminal region we of to 23 residues the C-terminal of GroEL chaperonin of chaperonin we the of of the C-terminal in the of of the of the of of the GroEL to of GroEL the C-terminal the C-terminal to the C-terminal residues the of GroEL in the of the of of the to in GroEL the of these to to the of of the C-terminal GroEL the of due to The of of the the of in the of chaperonin to the the the of the C-terminal region of GroEL the we the C-terminal the of the the C-terminal the to in to the of residues in to to GroEL the C-terminal to the of the the of the C-terminal of GroEL in GroEL residues the the C-terminal the we the the to the the C-terminal of chaperonin in the in the the we the the the the of in the we in the the to the the the C-terminal of the of C-terminal the the C-terminal region of the GroEL the the the of the of GroEL The of GroEL of GroEL in To the of the C-terminal region of GroEL the we the C-terminal the of the the C-terminal the to in to the of residues in to to GroEL the C-terminal to the of the the of the C-terminal of GroEL in GroEL residues the the C-terminal the we the the to the the C-terminal of chaperonin in the in the the we the the the the of in the we in the the to the the the C-terminal of the of C-terminal the the C-terminal region of the GroEL the the the of the of GroEL the of the GroEL to in the of we of in the of The The high GroEL The The to the of in the of are in are in in the of the the in the of chaperonin in the of the to the The of the in the of the of residues the of the the of these residues in the of the of in to the of residues the of of the to the of GroEL in the of high the of GroEL the chaperonin the to To the of the the we to in the of these of of the in the in the the The of to the of the in the to the of these to due to in the of the chaperonin the of the C-terminal the residues of the C-terminal in the of the GroEL the of to the chaperonin we of GroEL of the C-terminal in due to the the of in the of the GroEL in the to the of the the of of the of the 23 residues the of to the To the of the of we the of to The of to to of in the of GroEL the of the in the of the C-terminal region to the we the of the C-terminal region of the of the The of the GroEL probe to in of of in The of of in the of in to in to the in the we the in the of in the of 23 the the of the to the of in the these of the in the C-terminal region the in the to of GroEL of the in the C-terminal probe the the of the the of the C-terminal region in the region in the in of the GroEL region 23 residues are the C-terminal of C-terminal region of GroEL the The of residues are the GroEL GroEL The to the residues are to the of the region in chaperonin we GroEL to in the of to to the to in the of the To the of in the of in to to of to of the to the in of the the we coli GroEL of the of to in in coli of in of of in The of of in the of in the of of the The of to are in of of coli to the of the of region chaperonin we of the chaperonin in in of to of GroEL in to GroEL in coli in the of region chaperonin of in of of in the of are in of of coli GroEL the of the GroEL to in the of we of in the of The The high GroEL The The to the of in the of are in are in in the of the the in the of chaperonin in the of the to the The of the in the of the of residues the of the the of these residues in the of the of in to the of residues the of of the to the of GroEL in the of high the of GroEL the chaperonin the to To the of the the we to in the of these of of the in the in the the The of to the of the in the to the of these to due to in the of the chaperonin the of the C-terminal the residues of the C-terminal in the of the GroEL the of to the chaperonin we of GroEL of the C-terminal in due to the the of in the of the GroEL in the to the of the the of of the of the 23 residues the of to the To the of the of we the of to The of to to the C-terminal region to the we the of the C-terminal region of the of the The of the GroEL probe to in to the in the we the in the of in the of 23 the the of the to the of in the these of the in the C-terminal region the in the to of GroEL of the in the C-terminal probe the the of the the of the C-terminal region in the region in the in of the GroEL region 23 residues are the C-terminal of C-terminal region of GroEL the The of residues are the GroEL GroEL The to the residues are to the of the region in chaperonin we GroEL to in the of to to the to in the of the To the of in the of in to to of to of the to the in of the the we coli GroEL of the of to in in coli of in of of in The of of in the of in the of of the The of to are in of of coli to the of the of region chaperonin we of the chaperonin in in of to of GroEL in to GroEL in coli in the of region chaperonin of in of of in the of are in of of coli To the C-terminal region to the we the of the C-terminal region of the of the The of the GroEL probe to in to the in the we the in the of in the of 23 the the of the to the of in the these of the in the C-terminal region the in the to of GroEL of the in the C-terminal probe the the of the the of the C-terminal region in the region in the in of the GroEL region 23 residues are the C-terminal to the of the region in chaperonin we GroEL to in the of to to The the to in the of the To the of in the of in to to of to of the to the in of the the we coli GroEL of the of to in in coli to the of the of region chaperonin we of the chaperonin in in of to of GroEL in to GroEL in coli in the of region chaperonin C-terminal 23 residues of chaperonin GroEL are crystallographic due to high The role of C-terminal region in chaperonin are the C-terminal 23 residues in the to the role of these residues in chaperonin we generated C-terminal in the of the region in we generated of to the in chaperonin The of the of these GroEL in of GroEL the of the in of the of the of residues the C-terminal region in chaperonin of the C-terminal of chaperonin to the in the chaperonin The of the in the chaperonin of the the the of in the the we the C-terminal region role to the of the the C-terminal of residues of coli GroEL residues to we the of region in in in the in the to of The the role of region the of the of in the of the in in the of the to in the of in the of the to in coli in the of the C-terminal region of GroEL in of the chaperonin the of the of the of the in in The to the of the chaperonin to the of residues in the of the GroEL the of residues in The of residues the of the GroEL in of residues to residues in in the of the of the of the the of in the of the the are in the GroEL in in these residues of C-terminal to in to region in the of the chaperonin to region of the to the chaperonin the of the the of the in in the of GroEL we to the the GroEL C-terminal region the of the the in the of the GroEL C-terminal are in the of the The of these in the chaperonin mechanism, to the of these of we the of in to the of residues the the GroEL the of the of to to the in to the of the the in the of in in of the of in GroEL the the of the the of the of the C-terminal region are the of GroEL role the C-terminal region the GroEL in chaperonin in the of the of the of in the in of in the the the the of the the in the of the C-terminal the in the of the in of of we the C-terminal region role in the in the in the in the The C-terminal 23 residues of chaperonin GroEL are crystallographic due to high The role of C-terminal region in chaperonin are the C-terminal 23 residues in the to the role of these residues in chaperonin we generated C-terminal in the of the region in we generated of to the in chaperonin The of the of these GroEL in of GroEL the of the in of the of the of residues the C-terminal region in chaperonin of the C-terminal of chaperonin to the in the chaperonin The of the in the chaperonin of the the the of in the the we the C-terminal region role to the of the the C-terminal of residues of coli GroEL residues to we the of region in in in the in the to of The the role of region the of the of in the of the in in the of the to in the of in the of the to in coli in the of the C-terminal region of GroEL in of the chaperonin the of the of the of the in in The to the of the chaperonin to the of residues in the of the GroEL the of residues in The of residues the of the GroEL in of residues to residues in in the of the of the of the the of in the of the the are in the GroEL in in these residues of C-terminal to in to region in the of the chaperonin to region of the to the chaperonin the of the the of the in in the of GroEL we to the the GroEL C-terminal region the of the the in the of the GroEL C-terminal are in the of the The of these in the chaperonin mechanism, to the of these of we the of in to the of residues the the GroEL the of the of to to the in to the of the the in the of in in of the of in GroEL the the of the the of the of the C-terminal region are the of GroEL role the C-terminal region the GroEL in chaperonin in the of the of the of in the in of in the the the the of the the in the of the C-terminal the in the of the in of of we the C-terminal region role in the in the in the in the