A Rapid Assay of Suramin in Plasma

Abstract

Abstract The polysulfonated napthylurea suramin is currently undergoing extensive clinical trials. A narrow therapeutic window requires frequent dose adjustment to minimize toxicity. A simple, rapid and reproducible assay was developed for measuring suramin levels in plasma. Plasma samples are injected directly onto the column and eluted isocratically with acetonitrile and an ion pairing reagent. Retention time is approximately two minutes, recovery greater than 95% throughout the range of 50–300 ug/ml, with sensitivities to concentrations of 5 ug. A small sample size (0.5 ul) gave similar results allowing assays of finger-stick, samples. Equal accuracy was abtaiaedusingtrypanblueasaninterndlstandaril. careful d-, maWabxy for safe ncaninistmtion of the agent, can be acccilplisbed rapidly and accurately.