Constraints on the Structure of (CUG)₉₇ RNA from Magic‐Angle‐Spinning Solid‐State NMR Spectroscopy

Abstract

Twists in a spin: RNA structural constraints have been obtained directly by exploiting the dependence of inter- and intranucleotide 1H–1H interactions on RNA conformation through the use of 13C MAS solid-state NMR spectroscopy. The data provide evidence that (CUG)97 RNA principally adopts an A-form helix conformation with a C3′-endo sugar pucker and an anti glycosidic torsion angle χ (see model).