Angewandte Chemie International Edition · 2005 · 152 citations · 26 references
Life SciencesBiochemistryChemistryProtein BiochemistrySpectroscopyStructural BiologyMacromolecular Structure DeterminationProtein CharacterizationProtein ChemistryProtein ConformationProtein StructureNuclear Magnetic Resonance Structure DeterminationProtein Structural AnalysisProtein Structure AnalysisProtein Structure DeterminationThree-dimensional Nmr Spectroscopy
The three-dimensional fold of an immobilized polypeptide is predicted at atomic resolution from solid-state NMR spectroscopic data (see picture) obtained for a single uniformly [13C,15N] isotope labeled sample. The method involves the combined analysis of conformation-dependent NMR frequencies and short 1H–1H distances and may lead to the rapid characterization of three-dimensional protein structures.
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