Concepedia

In-gel detection of biotin–protein conjugates with a green fluorescent streptavidin probe

Alanna E. Sorenson, Samuel P. Askin, Patrick M. Schaeffer

Analytical Methods · 2015 · 26 citations · 24 references

DOIFull text

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Concepts

Abstract

A simple quantitative in-gel detection system was developed for measuring production of biotin–protein conjugates using a green fluorescent streptavidin probe.

References

24

[5] Avidin and streptavidin

N. Michael Green · Methods in enzymology on CD-ROM/Methods in enzymology · 1990 · 1K citations

, , +3

A Spectrophotometric Assay for Avidin and Biotin Based on Binding of Dyes by Avidin

N. M. Green · Biochemical Journal · 1965 · 442 citations

, , +4

Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin

Mark Howarth, Alice Y. Ting · Nature Protocols · 2008 · 265 citations · Full text

, , +13

[26] Biotinylation of proteins in vivo and in vitro using small peptide tags

Millard G. Cull, Peter J. Schatz · Methods in enzymology on CD-ROM/Methods in enzymology · 2000 · 234 citations

, , +12

Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation

Yifeng Li, Rui Sousa · Protein Expression and Purification · 2012 · 84 citations

, , +12