Differences in enzyme activities of <i>Lipomyces starkeyi</i> between cells accumulating lipid and proliferating cells

Abstract

Abstract Differences in intracellular enzyme activities between cells accumulating lipid and proliferating cells were studied with Lipomyces starkeyi , a fat‐producing yeast. When the cells were removed from a medium for lipid accumulation to a medium for cell proliferation, activities of several enzymes on the glycolytic pathway, pentosephosphate cycle, glycerol 3‐phosphate pathway, tricarboxylic acid cycle and fatty‐acid biosynthesis pathway changed. In the proliferating cells, activities of glycerol‐3‐phosphate dehydrogenase (GDH) and ATP citrate lyase were very low, as compared to those in the cells accumulating lipids. Activities of these two enzymes and 6‐phosphogluconate dehydrogenase, particularly GDH, decreased with the addition of zinc ion to the cell‐free extract of the cells accumulating lipid.