PLANT PHYSIOLOGY · 1967 · 108 citations · 6 references
Life SciencesPlant BiologyPlant SciencesPlant BiochemistryPlant Cell BiologyPlant PhysiologyProtein AnalysisProtein PurificationSeed AnatomySeed BiochemistryAnimal ProteinsCotyledon AnatomyCotyledon MorphologyProtein BodiesSeed Phytic AcidSucrose Gradient Centrifugation
Some microscope observations of the protein bodies of the cotyledon cells of the soybean (Glycine max) are described, together with changes in their appearance which occur on germination. Density gradient centrifugation permits the isolation of protein bodies from soymeal. They contain about 70% of the protein of the bean. Only 1 protein could be detected in them: glycinin, the major soybean protein.The protein bodies were fractionated to light and heavy fractions. The former contained 97.5% protein, the latter 78.5%. RNA, phytic acid and lipids were also present. The 2 fractions probably differ only in the extent of contamination by other cell fragments.
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Intracellular Distribution of Proteins in Pea Cotyledons
Joseph E. Varner, G. Schidlovsky · PLANT PHYSIOLOGY · 1963 · 95 citations · Full text